Reporter

Part:BBa_K763006:Design

Designed by: Luisa María Martínez Sánchez   Group: iGEM12_Valencia_Biocampus   (2012-09-17)

pTRR + Gene encoding ZsGreen1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal XhoI site found at 412
    Illegal XhoI site found at 946
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal NgoMIV site found at 7
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 44


Design Notes

No design considerations needed.


Source

The sequence of the promoter comes from yeast Sacharomyces cerevisiae genome, whereas the sequence encoding the ZsGreen1 fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.

References

1. Lee, J., Godon, C., Lagniel, G., Spector, D., Garin, J., Labarre, J. and Toledano, B. (1999) Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. The journal of biological chemistry 23(274): 16040-16046.

2. Kuge, S., Jones, N. and Nomoto, A. (1997) Regulation of yAP-1 nuclear localization in response to oxidative stress. EMBO J 16(7):1710-20

3. Mulford, K.E. and Fassler, J.S. (2011) Association of the Skn7 and Yap1 transcriptiom factors in the Saccharomyces cerevisiae oxidative Stress response. Eukaryotic cell 10(6):761